V. Ordeanu 1, V.A. Voicu 1, Gh. Ţarălungă 1, M. Necşulescu 1, Angela Postoarcă 1, Diana Popescu 1, Lucia E. Ionescu 1, Simona N. Bicheru 1, Cristina Secară 1, B. Patranichi 1
1 Army Center for Medical Research, Bucharest
Abstract
The study presents a concise selection of applicative research of pharmaceutical microbiology for the purpose of testing the biological activity of certain new thioureides having potentially anti-infectious effects. Objectives: the synthesis, the physical-chemical analysis and biological testing of the thioureides of the 2-(4-ethyl-phenoxymethyl)-benzoic acid (3 series), screening on aerobic bacteria, anaerobic bacteria, viruses, fungi, yeasts, spores and toxins, testing the anti-microbial effect according to the current European norms, acute toxicological testing on laboratory animals, comparing the results and statistical processing for the classification in pharmacological groups. The majority of the tested amides presented anti-bacterial effect; however, this was unequal, varying from very weak to very good; we deemed very weak the average effect occurring by the inhibition area having a diameter smaller than or equal to 6 mm (i.e. the substance application area), weak, good equivalent to the standard sample and very good higher than this value. We registered 6 substances having an effect. The effect is better on the Gram-positive bacteria, rather than on the Gram-negative ones, almost double, which could lead us to conclude that the effect would take place via the alteration of the bacterial wall structure, probably through the inhibition of the peptidoglycan synthesis. On average, the value of the diameter of the inhibition area of thioureides batch 2 as weighed against the standard bacteria tested, as compared with the mean for the phenol sample 5%. The compounds tested do no indicate antifungal, antiviral, sporicidal or anti-toxin effect.