Liliana Tarţău 1, Călin Andriţoiu 1, Elena Teslariu 2, Corina Dima 3, Eusebiu Viorel Şindrilar 4
1 Pharmacology – Algesiology Department, Gr.T. Popa University of Medicine and Pharmacy, Iaşi, Romania
2 Ocupational Health Department, Gr.T. Popa University of Medicine and Pharmacy, Iaşi, Romania
3 Internal Medicine, Recuperare Hospital Gr.T. Popa University of Medicine and Pharmacy, Iaşi, Romania
4 Anatomy Department, University of Agricultural Sciences and Veterinary Medicine, Iaşi, Romania
Abstract
Experimental researches on the eff ects of a selective k opioid receptor agonist in cutaneous and visceral pain models in mice. Material and method Th e experiments were carried out on white Swiss mice (20-25g), divided into 4 groups of 7 animals each, treated intraperitoneally with the same volume of solution, as follows: Group I: distilled water (Control) 0,3ml; Group II (U-50488H 10): U-50488H 10mg/kbw; Group III (U-50488H 20): U-50488H 20mg/kbw; Group IV (MOR): morphine 2mg/ kbw. Experimental protocols were implemented in accordance to the recommendations of the committee of research and ethics of the International Association for the Study of Pain. Th e nociceptive cutaneous testing was performed using the tail fl ick assay. Th e model of visceral pain consisted of infl ammatory cystitis after intraperitoneal injection of cyclophosphamide (200 mg/kbw). Th e data were presented as +/- SD and signifi cance was tested by SPSS for Windows version 13.0 and by the ANOVA method, followed by the Neumann Keuls test as post hoc. Results and conclusions. In our experimental conditions, U50,488H (10mg/kbw) determined antinociceptive signifi cant eff ects in tail fl ick test, 30 minutes after thermal noxious stimulation, but did not infl uence visceral nociceptive responses in cyclophosphamideinduced cystitis. Intraperitonal administration of selective k opioid agonist U50,488H, 20mg/kbw, resulted in a potent analgesia in both cutaneous and visceral pain models.