Galeș Laurenția 1, Anghel Rodica 1, Gruia Maria Iuliana 1, Negoiță Valentina 1
Vasilescu Monica 1, Panait Marieta 1, Bărbos D. 2, Gruia I. 3
1 Institute of Oncology “Prof. Dr. Alex. Trestioreanu” Bucharest – Romania
2 Nuclear Research Centre, Pitești, Romania
3 University of Bucharest, Faculty of Physics, Bucharest, Romania
Abstract
The angiogenesis process represents one of the most optimised biological processes in the human body. With our experiment we aimed to explore the oxidative stress route as enabler of angiogenesis inducing signal pathways. We materialized this scenario by using a lot of experimentally induced RS-1 hepatoma cells carrier animals that we treated with an angiogenesis inhibitor and, in a later phase, with BNCT irradiation. The serum was processed by centrifugation in view of determining the oxidative stress parameters, while cells from the tumour tissue were collected with the purpose to establish apoptosis through flow-cytometry. Results obtained from flow cytometry measurements indicate the occurrence of apoptosis after a second course of Bevacizumab. After a first round of Bevacizumab, apoptosis increased from 9.12% to 20.14% but dropped to 12.05% following BNCT administration. These results highlight an expansion in the oxidative metabolism, at the level of the tumour tissue, compared to values registered after serum determination. The administration of an anti-angiogenic compound triggers lower oxygen and nutrients flow thus determining a slower lipid peroxidation reaction. Even in the context of oxygen intake limitation it turned out that the albumin thiols’ value in the tumour tissue augments. Ceruloplasmin is not expressed in the murine tumour tissue. The obtained values lead to several conclusions, namely: oxygen reactive species are secondary signalling messengers for the initiation of vital biochemical reactions; when produced in small quantities they will have an active participation in the signalling cascade but when they come in large concentrations they become destructive effectors and thus can be used in the monitoring of radiation therapy.