Drăghia F. 1, Drăghia Alina 2, Păunescu H. 3, Coman L. 4, Gaiu Irina 5, Bădărau Anca 4
1 Department of Anatomy, University of Medicine and Pharmacy “Carol Davila” Bucharest
2 Department of Cellular and Molecular Medicine, University of Medicine and Pharmacy “Carol Davila” Bucharest
3 Department of Pharmacology and Pharmacotherapy, University of Medicine and Pharmacy “Carol Davila” Bucharest
4 Department of Physiology, University of Medicine and Pharmacy “Carol Davila” Bucharest
5 Student, University of Medicine and Pharmacy “Carol Davila” Bucharest
Abstract
The present study tried to determine the variations in the enzymatic, oxidoreductive and aerobic activity of the NADH2-cytochrome-C-reductase (diaphoresis) by histochemical methods, in comparison to that of the glycolic anaerobic enzyme (lactate dehydrogenase), using an experimental model of poisoning with carbon tetrachloride (CCl4) in rats. The liver was investigated at 24 hours after poisoning. Two standardized histochemical techniques were used. Intensity of reaction, the parameter used, was determined by qualitative evaluation of color, as visualized with optical microscope. The diaphorase presented an intense and very intense decrease of reaction in vascular endothelia and in hepatocytes .In hepatocytes, the decrease of the enzymatic, oxidative and aerobic activity appeared especially in the pericentrolobular area. The intensity of reaction was unequal in middle area of the lobular cells and maintained its intensity in the periportal biliary hepatocytes. The lactate dehydrogenase presented different changes from a liver lobule to another, either by highly diminishing of reaction in the hepatocytes of the entire lobule, or by diminishing of reaction unequal from a hepatocyte to another in the same lobule. In some liver lobules appeared modifications in the metabolism of the hepatic lobule for both of the studied oxidative enzymes. It was more obvious for the lactate dehydrogenase. There were also noticed some structural modifications, with cellular necrosis and/or numerous lipid drops in the cytoplasm. The observations and the results of the experimental intoxication emphasize the noxious consequences which some pesticides will have on the organism, expressed on the liver in the present study.