Galeș Laurenția 1, Gruia Maria Iuliana 1, Negoiță Valentina 1, Vasilescu Monica 1, Panait Marieta 1, Barbos D. 2, Gruia I. 3, Anghel Rodica 1
1 Institute of Oncology “Prof. Dr. Alex. Trestioreanu” Bucharest, Romania
2 Nuclear Research Center Pitesti Romania
3 University of Bucharest, Faculty of Physics, Bucharest-Romania
Abstract
The purpose of this study is to identify some molecular mechanisms which occur after the boron neutron capture therapy has been applied to experimental hepatoma cells, compared with the normal surrounding tissues. The purpose is to obtain useful data that could improve the biological and biochemical treatment response. Material and methods: We used tumor cells from a chemically induced experimental hepatoma at Wistar rats. The tumor and normal tissue were removed in the dynamic of the growth and development of the tumor, (14, 21, 28, 35 and 42 days after the inoculation). From these tissues we isolated cells and we performed the determination of cell cycle phases, apoptosis and free radicals of oxygen levels. The cells were then irradiated in the prompt gamma device placed at the radial channel of the TRIGA-ACPR reactor from Pitesti-Romania. After the irradiations we reassessed the same parameters. Results and discussions: Our results show an increase of oxidative stress monitored at a h igher level f rom t he investigated parameters’ values. The cells were not identified in t he G0 phase of the cellular cycle, instead we identified apoptotic cells (the apoptosis rate increase to 54.75% and 70.42% after 1800 and 2700 seconds irradiation respectively, compared to an initial value of 11.48%), this suggesting the initiation of irreversible bond breakage, irreparable, mediated by oxygen free radicals.