EVALUAREA COMPARATIVĂ A SUBPOPULAŢIILOR LIMFOCITARE T ÎN SÂNGELE PERIFERIC ŞI ÎN PARENCHIMUL HEPATIC, ÎN HEPATITELE CRONICE CU HBV ŞI HCV

Carmen Ardeleanu¹, M. Ceaușu², C. Ursaciuc³, Florina Vasilescu⁴, Cristina Iosif⁵, Florica Stăniceanu⁶, Camelia Vrabie⁷, Maria Sajin⁸, Dan Butur⁹, Georgeta Butur¹⁰
1. Conf. Dr. CPI Carmen Ardeleanu, UMF “Carol Davila”, INCD „Victor Babeş” Bucureşti
2. As. Univ. Dr. CS Mihai Ceaşu, UMF “Carol Davila”, INCD „Victor Babeş” Bucureşti
3. Dr. CPII Cornel Ursaciuc, INCD „Victor Babeş”, Bucureşti
4. Dr. Florina Vasilescu, INCD „Victor Babeş” Bucureşti, SMC-Serviciul Anatomie Patologică, Bucureşti
5. Dr. Cristina Iosif, INCD „Victor Babeş” Bucureşti, Sp. Sfânta Maria”, Bucureşti
6. Prof. Dr Florica Stăniceanu, UMF “Carol Davila”, Sp. Clinic Colentina – Serviciul Anatomie Patologică, Bucureşti
7. Dr As. Univ. Camelia Vrabie, Sp. Clinic „Sf. loan”, UMF “Carol Davila” Bucureşti
8. Conf. Dr. Maria Sajin, UMF „Carol Davila”, INCD “Victor Babeş”, Bucureşti
9. Bioch. Dan Butur, INCD „I. Cantacuzino”, Bucureşti
10. Biol. CPIII Georgeta Butur, INCD „Victor Babeş” Bucureşti

Abstract

Background: T lymphocytes population are hypoth­ ized to be involved in the evolution and immunopatho­es genesis of CVH, since they control and eliminate viruses from infected cells, directly through CD8+ T lymphocytes and indirectly by CD4+ T lymphocytes. Aim: Comparative assessment of T lymphocytes sub­ populations in peripheral blood and hepatic parenchyma in patients infected with HBV and HCV. Material and methods: CD4+ and CD8+ cells were assessed by flow cytometry and immunohistochemistry methods in 15 patients with chronic B hepatitis and 38 patients with chronic C hepatitis serologically confirmed. Results: In peripheral blood, CD4+ T lymphocytes had increased values in B virus chronic hepatitis (70% of patients) and normal I slightly increased values in C virus chronic hepatitis (48% and 39% of patients). CD8+ lymphocytes presented a significant decrease in both types of chronic viral infections (over 80% of patients). In hepatic parenchyma, quantitative analysis of CD4+ lymphocytes emphasized a low number of cells, especially for HBV chronic hepatitis (90% of patients). In HCV chronic hepatitis, an increased number of CD4+ cells were ob­ served in over 40% of cases. Quantitative assessment of CD8+ lymphocytes revealed an increased number of cells in chronic infections with HBV (50% of patients), while it was decreased in HCV infections (80% of patients). Conclusion: The imbalance of lymphocytes subpopu­ lations, especially CD8+ cells in peripheral blood may be the expression of a weak antiviral immune response, with a corresponding inability to eradicate virus. This immune deviation facilitates liver damage, especially in HCV infections.