Petru Scripcariu *, Constantin Cotrutz **, O. C. Mungiu **, Sultana Mihailovici , Carmen Cotrutz*, Elena Teslaru ***, Olga Scripcariu *, Emil Jacob ****, Dan Despa ****, Cornel Moșoiu **, Tudor Petreuș **, Cezar Ionescu **, Adrian Cosovanu
* Petru Scripcariu, Olga Scripcariu – Clinica Urgențe Medicale Iași
** Constantin Cotrutz, Carmen Cotrutz, Cornel Moșoiu, Tudor Petreuș, Cezar Ionescu – Catedra Biologie Celulară, UMF Iași
*** O.C. Mungiu, Elena Teslaru – Catedra Farmacologie, UMF lași
**** Emil Jacob, Dan Despa – Institutul de Sănătate Publică Iași
***** Adrian Cosovanu – Clinica a Ill-a Medicală Cardiologică I. Enescu, UMF Iași
****** Sultana Miltailovici – Catedra de Anatomie Patologică, UMF Iași
Abstract
Ethanol (20 mM/1 and 200 mM/I) and acethaldehide (10 mm/I and 100 mM/1) exposure results in more than 95% detachment from substrate of rat cultured cardiomyocites. On light microscope we didn’t notice any cellular changes on the cultured cardiomyocites exposed to ethanol. But on the cardiomyocites exposed to acethaldehide we have noticed small cytoplasmatic vacuoles that resulted from the disappearance of PAS positive substance (glicogen). Neither on electron microscope we didn’t notice any change of cells exposed to ethanol. But on the cardiomyocites exposed to acethaldehide we noticed a mithocondrial swollen and an intensification of the functional activity of the endoplasmatic reticulum, manifested as formation of the transit microvesicules. The study reveals that harmful acute effects of the ethanol at the cardiomyocites level is exercited especially through his toxic metabolite acethaldehide, the ethanol having as impact point the cytoplasmatic membrane.