Liliana Tarţău 1, E. V. Şindrilar 2, R. V. Lupuşoru 3, Cătălina Elena Lupuşoru 1
1 Pharmacology – Algesiology Department, „Gr.T. Popa” University of Medicine and Pharmacy, Iaşi, Romania
2 Anatomy Department, University of Agricultural Sciences and Veterinary Medicine, Iaşi, Romania
3 Pathophysiology Department “Gr.T. Popa” University of Medicine and Pharmacy, Iaşi, Romania
Abstract
Aim: Experimental researches on the effects of certain 5-HT3 serotonin receptor antagonists in visceral pain models in mice. Material and method: The experiments were carried out on white Swiss mice (20-25g), divided into 4 groups of 7 animals each, treated intraperitoneally with the same volume of solution, as follows: Group I (Control): distilled water 0,3ml; Group II (OND): ondansetron 1mg/kbw; Group III (TRO): tropisetron 0,5mg/kbw; Group IV (PAL): palonosetron 4μg/kbw. Th e experimental protocols were implemented according to the recommendations of the committee of research and ethics of the International Association for the Study of Pain. The models of visceral pain consist of writhing test with acetic acid 0,6% and colon inflammation test with capsaicin. Intraperitoneal injection of the irritant agent acetic acid causes a typical stretching response named writhing. Intracolonic administration of capsaicin triggered visceral pain-related nociceptive behavioral manifestations (licking the abdomen, stretching, contractions). In both tests, characteristic behavioral manifestations were observed, scored and the data was statistically analyzed. The data was presented as +/- SD and significance was tested by SPSS for Windows version 13.0 and by ANOVA method, followed by Neumann Keuls test as post hoc. Results and conclusions: In our experimental conditions, serotonin 5-HT3 subtype receptor antagonist ondansetron, determined analgesic eff ects in both writhing test and colon inflammation model. 5-HT3 receptor antagonists- tropisetron and palonosetron did not significantly influence nociceptive responses through chemical peritoneal irritation or colon inflammation.